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Modulation of hybridoma cell growth and antibody production by coating cell culture material with extracellular matrix proteins

Paper ID Volume ID Publish Year Pages File Format Full-Text
4704 239 2007 8 PDF Available
Title
Modulation of hybridoma cell growth and antibody production by coating cell culture material with extracellular matrix proteins
Abstract

The influence of coating polystyrene tissue culture plates with different proteins on murine hybridoma cell growth and antibody production was investigated. Fibronectin, collagen I, bovine serum albumin and laminin were used to coat NUNC® and COSTAR® cell culture plates. Cell number and antibody concentration in culture fluids were quantified as indicators for cell viability, proliferation and productivity. Adhesive behaviour, morphology, expression of surface receptors of hybridoma cells and the presence of tyrosine-phosphorylated proteins in cell lysates were characterized by cell adhesion experiments, microscopy, flow cytometry and Western Blot analysis.It was shown that coatings with fibronectin (0.2 μg/ml) lead to a substantial improvement of cell growth by 50–70% and an increase of monoclonal antibody production by 100–120%.Collagen I coatings showed an improvement in cell growth by 30–70% and by 60% for the production of monoclonal antibodies. Coatings with BSA and laminin had minor effects on these parameters. It was found that the hybridoma cell lines used in this study did not express the α2-chain of the α2β1-integrin, which is responsible for binding to collagen and laminin.However, the presence of β1-integrin on the cell surface was shown, which should enable hybridoma cells to bind fibronectin. We propose, therefore, that fibronectin adsorption to cell culture materials may be a promising approach to enhance the production of monoclonal antibodies by cultivated hybridoma cells.

Keywords
Hybridoma cells; Tissue culture plates; Cell growth; Antibody production; Extracellular matrix; Fibronectin adsorption
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Modulation of hybridoma cell growth and antibody production by coating cell culture material with extracellular matrix proteins
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 35, Issue 3, 1 August 2007, Pages 301–308
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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