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Separation of l-lysine from dilute aqueous solution using molecular imprinting technique

Paper ID Volume ID Publish Year Pages File Format Full-Text
4710 239 2007 5 PDF Available
Title
Separation of l-lysine from dilute aqueous solution using molecular imprinting technique
Abstract

In the present study, separation of l-lysine from dilute aqueous solution by solid-phase extraction based on molecular imprinting technique using a polar porogen was investigated. l-Lysine imprinted polymer (LLIP) was prepared by free radical solution polymerization of methacrylic acid and ethylene glycol dimethacrylate as functional and cross-linking monomers, in the presence of l-lysine as an imprint molecule, mixture of water and methanol as solvent and AIBN as an initiator. Non-imprinted polymer (NIP) as control was also prepared by the same procedure in the absence of template molecules. LLIP particles were applied to determine the optimum operational condition for l-lysine separation from dilute aqueous solution. In adsorption step, optimum pH and retention time were 7.8 and 90 min, while corresponding values in extraction step were 12 and 50 min, respectively. l and d-Lysine recovery by LLIP at optimum condition were found to be 96 and 58% with corresponding distribution coefficients of 8000 and 460, respectively. The retention capacity of LLIP was 27.26 mg l-lys/g of polymer at optimum condition.

Keywords
Molecular imprinting; Lysine; Separation; Adsorption; Optimum condition; Retention capacity
First Page Preview
Separation of l-lysine from dilute aqueous solution using molecular imprinting technique
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 35, Issue 3, 1 August 2007, Pages 352–356
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering