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Environmental stimuli on the soluble expression of anti-human ovarian carcinoma × anti-human CD3 single-chain bispecific antibody in recombinant Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
4726 240 2007 8 PDF Available
Title
Environmental stimuli on the soluble expression of anti-human ovarian carcinoma × anti-human CD3 single-chain bispecific antibody in recombinant Escherichia coli
Abstract

The influences of different environmental stimuli on the soluble expression of anti-human ovarian carcinoma × anti-human CD3 single-chain bispecific antibody in Escherichia coli were examined to improve its yield. Often, it was found that the amount ratio of the inclusion body fraction to the soluble fraction of the recombinant protein varied greatly with the changes of environmental conditions in fermentation process. Wherefore, an improved strategy based on various undercritical stimuli, including such environmental factors as temperature-shift, pH-shift and repetitious pH-shift, was employed to enhance the soluble expression of the target protein. As a result, an optimal factor-shift process was determined and the soluble yield of the target protein increased by 82.9% (from 68.9 to 126.6 mg protein/g DCW) in flask fermentation compared with the result cultured at 30 °C. And the high soluble expression level was also obtained in a 3.7 L fermentor based on this strategy. This improved strategy that factor-shifts under undercritical environmental stimuli improve the soluble expression of exogenous proteins in recombinant E. coli may become a new alternative besides the genetic engineering considerations.

Keywords
Anti-human ovarian carcinoma × anti-human CD3; Environmental stimuli; Factor-shift; Inclusion body; Recombinant E. coli; Soluble expression
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Environmental stimuli on the soluble expression of anti-human ovarian carcinoma × anti-human CD3 single-chain bispecific antibody in recombinant Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 37, Issue 2, 15 November 2007, Pages 184–191
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us