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Recovery and separation of surfactin from pretreated fermentation broths by physical and chemical extraction

Paper ID Volume ID Publish Year Pages File Format Full-Text
4739 241 2008 8 PDF Available
Title
Recovery and separation of surfactin from pretreated fermentation broths by physical and chemical extraction
Abstract

The recovery of surfactin from fermentation broths with the culture of Bacillus subtilis ATCC 21332 by physical and chemical extraction was studied, in which the broths were pretreated by acid precipitation and, if necessary, the precipitate was further dissolved in NaOH solution. The physical solid–liquid and liquid–liquid extractions were performed with different organic solvents (ethyl acetate, n-hexane) and at different times of extraction run. It was shown that better extraction was obtained with ethyl acetate than n-hexane. The extraction could be improved by increasing the times of extraction run under a given volume of the organic solution. For chemical liquid–liquid extraction of surfactin with Aliquat 336 (5–200 mM) in n-hexane, the efficiency was improved. The amounts of inorganic salt in the strip solution were also optimized. Surfactin recovered after physical or chemical extraction was finally characterized by mass and NMR spectroscopies. The results of chemical extraction showed that surfactin would readily bind with the quaternary ammonium cations of Aliquat 336.

Keywords
Surfactin; Recovery; Fermentation broths; Extraction; Ethyl acetate; n-Hexane; Aliquat 336
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Recovery and separation of surfactin from pretreated fermentation broths by physical and chemical extraction
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 38, Issue 1, 15 January 2008, Pages 39–46
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us