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Improvement of propagation frequency with two-step direct somatic embryogenesis from carrot hypocotyls

Paper ID Volume ID Publish Year Pages File Format Full-Text
4741 241 2008 6 PDF Available
Title
Improvement of propagation frequency with two-step direct somatic embryogenesis from carrot hypocotyls
Abstract

Mass production of the regenerated plantlets from carrot hypocotyls via two-step direct somatic embryogenesis (DSE) was investigated. Somatic embryos were induced in the induction culture with medium containing 2,4-dichlorophenocxyacetic acid (2,4-D) prior to the development culture in this method. The optimum condition for the regeneration efficiency was found to be 2 weeks of induction culture using hypocotyls shorter than 3 cm. The distribution profile of the regeneration efficiency corresponded to that of the normal distribution. The distribution range was observed to depend on the length of the hypocotyls. Calcium addition increased the regeneration efficiency but did not affect the distribution range. We also found that this procedure was applicable to the mass production of regenerated plants using a bioreactor.

Keywords
Direct somatic embryo; Carrot; Plant cell culture; Tissue cell culture; Plant cell bioreactor; Airlift bioreactor
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Improvement of propagation frequency with two-step direct somatic embryogenesis from carrot hypocotyls
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 38, Issue 1, 15 January 2008, Pages 55–60
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us