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Kinetic stabilities of soybean and horseradish peroxidases

Paper ID Volume ID Publish Year Pages File Format Full-Text
4748 241 2008 5 PDF Available
Title
Kinetic stabilities of soybean and horseradish peroxidases
Abstract

Peroxidases have attractive biocatalytic properties and are used in biosensing and immunoassays. Among various peroxidases, isoenzyme C of horseradish peroxidase (HRP-C) is the most studied, and is also the most commercially used due to its high structural stability. Soybean peroxidase (SBP) and horseradish peroxidase share strikingly similar three-dimensional structures with ∼60% sequence homology. We reported previously, that the conformational and thermal stabilities of SBP are substantially higher than HRP-C. In the present study, we show that the kinetic stability of SBP is much higher than HRP-C as obtained by measuring their unfolding rates at various guanidine hydrochloride (GdnHCl) concentrations. In contrast, the heme-free forms of SBP and HRP-C showed similar kinetic stabilities. We conclude that the higher structural stability of SBP compared to HRP-C stems from the heme binding to the apo protein. Commercial interest of these results is twofold. A cheaper, abundant, better active, and more stable SBP could replace HRP-C. The stability and hence the biocatalytic property of a peroxidase can be improved by suitably engineering the heme active-site that enhances the heme-apo-protein interaction.

Keywords
SBP, soybean peroxidase; HRP-C, horseradish peroxidase isoenzyme C; GdnHCl, Guanidine hydrochloride (enclosing square brackets indicate concentration); ΔG(H2O), equilibrium free energy of unfolding in water; kU (H2O), unfolding rate constant in water; t1/
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Kinetic stabilities of soybean and horseradish peroxidases
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 38, Issue 1, 15 January 2008, Pages 110–114
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us