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Synthesis of thermo-sensitive polyacrylamide derivatives for affinity precipitation and its application in purification of lysozyme

Paper ID Volume ID Publish Year Pages File Format Full-Text
4775 244 2007 6 PDF Available
Title
Synthesis of thermo-sensitive polyacrylamide derivatives for affinity precipitation and its application in purification of lysozyme
Abstract

The thermo-sensitive N-alkyl substituted polyacrylamide polymer was synthesized by radical polymerization and its lower critical solution temperature (LCST) was controlled to be 28 °C. The thermo-sensitive recovery of polymer was over 95% in the presence of 0.05 M NaClO4. Cibacron Blue F3GA was covalently immobilized onto the polymer via the nucleophilic reaction between the active chlorine atom of its triazine ring and the hydroxyl group of the polymer. The ligands density was 30 μmol g−1 polymer. The adsorption capacity of lysozyme on the polymer was 3.4 mg g−1polymer in affinity precipitation process. And over 90% of adsorbed lysozyme was eluted by 0.5 M KSCN at pH 8.0. When the affinity polymer was applied in the purification of lysozyme from egg white, the purification factor was 28 and lysozyme yield was 80% or so.

Keywords
Thermo-sensitive; N-Alkyl substituted acrylamide polymer; Lower critical solution temperature; Affinity precipitation; Cibacron Blue F3GA
First Page Preview
Synthesis of thermo-sensitive polyacrylamide derivatives for affinity precipitation and its application in purification of lysozyme
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 33, Issue 1, January 2007, Pages 66–71
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering