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Thermolysis of recombinant Escherichia coli for recovering a thermostable enzyme

Paper ID Volume ID Publish Year Pages File Format Full-Text
4778 244 2007 5 PDF Available
Title
Thermolysis of recombinant Escherichia coli for recovering a thermostable enzyme
Abstract

The development of recombinant DNA has made it feasible to produce a wide range of valuable protein products in the bacterium Escherichia coli. Extraction of intracellular protein from E. coli is traditionally achieved by mechanical, chemical or enzymatic disruption technology. In this study, thermolysis, which differs from the traditional ones, is presented for disruption of E. coli cells to release recombinant thermostable enzyme. Heat treatment of E. coli at 80 °C is highly effective to destroy the integrity of the bacterial cell wall and release the recombinant thermostable enzyme. At the same time of disruption, the recombinant thermostable enzyme was partially purified. Moreover, thermolysis was carried out in fermentation broth in situ, which may make it a more applicable approach for industrial-scale processes.

Keywords
rpm, round per minute; IPTG, isopropyl-β-d-thiogalactopyranoside; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresisBioseparations; Cell risruption; Downstream processing; Protein recovery; Thermolysis; Escherichia coli
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Thermolysis of recombinant Escherichia coli for recovering a thermostable enzyme
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 33, Issue 1, January 2007, Pages 94–98
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us