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Purification of potato polyphenol oxidase (PPO) by partitioning in aqueous two-phase system

Paper ID Volume ID Publish Year Pages File Format Full-Text
4832 249 2006 6 PDF Available
Title
Purification of potato polyphenol oxidase (PPO) by partitioning in aqueous two-phase system
Abstract

The aim of this work is to extract and purify potato polyphenol oxidase (PPO) from the potato tuber (Solanum tuberosum) using aqueous two-phase system (ATPS). The PPO was extracted by partitioning in ATPS composed of polyethylene glycol (PEG) and phosphate. Effect of phase composition, molecular weight of PEG and pH of the system on enzyme partitioning and selective enrichment was studied. The optimum system was found at pH 7.0 containing 5% PEG-8000 and 28.5% phosphate, with Ka of 32.3, purification factor of 15.7 and a 97.0% yield of enzyme activity in the top phase.

Keywords
Aqueous two-phase systems; Polyphenol oxidase; Downstream processing; Protein recovery; Enzyme purification
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Purification of potato polyphenol oxidase (PPO) by partitioning in aqueous two-phase system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 28, Issue 2, 15 February 2006, Pages 161–166
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us