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Influence of the functional activating agent on the biochemical and kinetic properties of Candida rugosa lipase immobilized on chemically modified cellulignin

Paper ID Volume ID Publish Year Pages File Format Full-Text
4843 250 2007 7 PDF Available
Title
Influence of the functional activating agent on the biochemical and kinetic properties of Candida rugosa lipase immobilized on chemically modified cellulignin
Abstract

Candida rugosa lipase was immobilized by covalent binding on wood cellulignin (Eucaliptus grandis) chemically modified with different activating agents as carbonyldiimidazole, glutaraldehyde and sodium metaperiodate. The resulted immobilized derivatives were evaluated in both aqueous (hydrolysis) and organic (ester synthesis) media. In aqueous media a comparative study between free and immobilized derivatives was provided in terms of pH, temperature and kinetic constants (Vmax and Km) following the hydrolysis of p-nitrophenyl palmitate, in which new optima values were established. The experimental results suggested that functional activating agents render different interactions between enzyme and cellulignin, producing consequently alterations in the optimal reaction conditions. Different behavior was found when the immobilized derivatives were tested in organic media, under these conditions similar esterification activities were observed, independent on the agent used to active the immobilizing support. Reasons for this are discussed on the light of the interactions among the support, functional activating agent and lipase structure.

Keywords
Candida rugosa lipase; Cellulignin; Functional activating reagent; Immobilization
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Influence of the functional activating agent on the biochemical and kinetic properties of Candida rugosa lipase immobilized on chemically modified cellulignin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 34, Issue 1, April 2007, Pages 13–19
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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