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Modification with DEAE-dextran, an alternative way to prepare anion-exchange monolithic column with lower pressure drop

Paper ID Volume ID Publish Year Pages File Format Full-Text
4850 250 2007 6 PDF Available
Title
Modification with DEAE-dextran, an alternative way to prepare anion-exchange monolithic column with lower pressure drop
Abstract

Poly(methacrylate-co-ethylene dimethacrylate) monolithic column (100 mm × 4.6 mm i.d.) subsequently modified with DEAE-dextran had been prepared for the separation of proteins with anion-exchange mode. The column, which possessed the specific surface area of 5.3 m2/g and immobilized amine group of 9.93 μmol/g media, showed even better permeability of 607 psi at flow rate of 1445 cm/h in comparison to diethylamine modification procedure. The dynamic binding capacity of bovine serum albumin (BSA) was 23.8 mg/g media. Fast separation of lysozyme, hemoglobin and BSA on the column was achieved within 3 min at flow rate of 1445 cm/h.

Keywords
Monolithic column; DEAE-dextran; Protein; Separation
First Page Preview
Modification with DEAE-dextran, an alternative way to prepare anion-exchange monolithic column with lower pressure drop
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 34, Issue 1, April 2007, Pages 76–81
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering