fulltext.study @t Gmail

Partitioning of model proteins in aqueous two-phase systems containing polyethylene glycol and ammonium carbamate

Paper ID Volume ID Publish Year Pages File Format Full-Text
4853 250 2007 6 PDF Available
Title
Partitioning of model proteins in aqueous two-phase systems containing polyethylene glycol and ammonium carbamate
Abstract

The phase equilibrium and the partitioning of bovine serum albumin, trypsin and lysozyme in aqueous two-phase systems containing ammonium carbamate and polyethylene glycol 1500, 4000 and 6000 are presented in this communication. Partition coefficients, defined as the ratio of protein concentration in the polymer phase to that in the salt phase, vary in the range of 0.1–0.8 for bovine serum albumin, 1.0–2.4 for trypsin and from 2.3 to 9.0 for lysozyme, depending on the polymer chain size and on the tie-line length. The analysis of the separation factor, defined as the ratio of partition coefficients of two proteins, shows that high degrees of separation can be achieved. These results indicate that aqueous two-phase systems containing ammonium carbamate and polyethylene glycol can be an alternative to be considered for protein downstream processing.

Keywords
Protein; Downstream processing; Aqueous two-phase systems; Salt; Polymer
First Page Preview
Partitioning of model proteins in aqueous two-phase systems containing polyethylene glycol and ammonium carbamate
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 34, Issue 1, April 2007, Pages 92–97
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us