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Use of chitosan–clay composite as immobilization support for improved activity and stability of β-glucosidase

Paper ID Volume ID Publish Year Pages File Format Full-Text
4867 251 2007 6 PDF Available
Title
Use of chitosan–clay composite as immobilization support for improved activity and stability of β-glucosidase
Abstract

Equal weights of cuttlebone chitosan and activated clay were mixed and prepared as the wet (without freeze-drying) and dried (with freeze-drying) beads. The resulting composite beads were then cross-linked with glutaraldehyde as the supports for β-glucosidase immobilization. The immobilization conditions including enzyme loading, immobilization time, cross-linking time, and the amount of glutaraldehye were optimized. The properties of the enzyme immobilized on wet and dried composites were compared. It was shown that the storage stability of immobilized enzyme was higher than that of free enzyme. The activity of immobilized enzyme on dried composite was higher those on wet chitosan beads and wet composite after being repeatedly used for 50 times. The pH and thermal stabilities of free and immobilized enzymes were also investigated.

Keywords
β-Glucosidase; Immobilization; Chitosan–clay composite; Stability
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Use of chitosan–clay composite as immobilization support for improved activity and stability of β-glucosidase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 35, Issue 1, 1 July 2007, Pages 93–98
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us