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Effect of aprotic solvents on the enzymatic activity of lipase in AOT reverse micelles

Paper ID Volume ID Publish Year Pages File Format Full-Text
4872 253 2006 8 PDF Available
Title
Effect of aprotic solvents on the enzymatic activity of lipase in AOT reverse micelles
Abstract

The modification of reverse micellar systems composed of AOT, isooctane, water by the addition of aprotic solvents has been performed. The impact of this change on the activity, stability and kinetics of solubilized Chromobacterium viscosum lipase (glycerol-ester hydrolase, EC 3.1.1.3) was investigated. Of seven aprotic solvents tested, dimethyl sulfoxide (DMSO) was found to be most effective. It was found that lipase activity was enhanced by optimizing some relevant parameters, such as water–AOT molar ratio (W0), buffer pH and surfactant concentration. A kinetic model that considers the free substrate in equilibrium with the substrate adsorbed on the micellar surface was successfully used to deduce some kinetic parameters (Vmax, Km and Kad), and the values of Km and Kad were significantly reduced by the presence of DMSO. Higher lipase stability was found in AOT reverse micelles with DMSO compared with that in simple AOT systems with half-life of 125 and 33 days, respectively. Fluorescence spectroscopy and Fourier transform infrared spectroscopy (FT-IR) were used to elucidate the effects of DMSO on the properties of AOT reverse micelles.

Keywords
Reverse micelles; Lipase; Hydrolysis; DMSO; Enzyme stability and kinetics
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Effect of aprotic solvents on the enzymatic activity of lipase in AOT reverse micelles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 30, Issue 3, 25 June 2006, Pages 237–244
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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Price after discount Only $4.95
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