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Run-to-run fed-batch optimization for protein production using recombinant Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
4877 253 2006 7 PDF Available
Title
Run-to-run fed-batch optimization for protein production using recombinant Escherichia coli
Abstract

A two-phase design approach is introduced to determine the optimal feed rate, fed glucose concentration and fermentation time to maximize protein productivity using recombinant Escherichia coli BL21 (pBAW2) strain. The first phase is applied to determine a primary S-system kinetic model using batch time-series data. Two runs were carried out in the second phase to achieve the maximum protein productivity for the fed-batch fermentation process. The computational results using the S-system kinetic model obtained from the second run are in better agreement with the experiments than those using the kinetic model obtained from batch time-series data. For cross-validation, two extra fed-batch experiments with different feed strategies were carried out for comparison with the optimal fed-batch result. From the experimental results, this approach could improve productivity by at least 3%.

Keywords
S-system; Optimization; Hybrid differential evolution; Fed-batch culture; Fermentation; Bioreactor
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Run-to-run fed-batch optimization for protein production using recombinant Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 30, Issue 3, 25 June 2006, Pages 279–285
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us