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Effect of two-steps substrate addition on steroids 11β-hydroxylation by Curvularia lunata CL-114

Paper ID Volume ID Publish Year Pages File Format Full-Text
4898 255 2006 6 PDF Available
Title
Effect of two-steps substrate addition on steroids 11β-hydroxylation by Curvularia lunata CL-114
Abstract

In this paper, the effect of substrate 17α-hydroxypregn-4-ene-3,20-dione-21-acetate (RSA, Cortexolone-21-acetate) on the expression of cytochrome P450 and the production of hydrocortisone by Curvularia lunata CL-114 was studied. Meanwhile the effect of pH on the production of hydrocortisone was observed. Based on the effect of substrate RSA two-steps addition on cytochrome P450 expression and hydrocortisone production, a novel fermentation process was established as follows: 0.3 g/L RSA was added for the first time after 16 h of inoculation, followed by the second addition of 0.7 g/L RSA after 8 h later, then pH was regulated to constant 6.5 after another 8 h till the end of fermentation. The results showed that the novel process was much better than the original one on improving the induction of cytochrome P450 and production of hydrocortisone, and the hydrocortisone yield had achieved an improvement of 17.6% higher than that of the original process correspondingly.

Keywords
Curvularia lunata; Hydrocortisone; Cytochrome P450; Steroid; Induction
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 32, Issue 3, 1 December 2006, Pages 233–238
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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