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Superporous pellicular agarose–glass composite particle for protein adsorption

Paper ID Volume ID Publish Year Pages File Format Full-Text
4903 256 2007 8 PDF Available
Title
Superporous pellicular agarose–glass composite particle for protein adsorption
Abstract

A superporous pellicular agarose–glass (SPAG) composite particle has been prepared by coating 6% agarose gel onto glass beads through water-in-oil emulsification using a mixture of cyclohexane and fine calcium carbonate granules as porogenic agent. After cross-linking, the cyclohexane was removed by proper solvent and the solid granules were removed by dissolving them in hydrochloric acid. Then, the composite particle was modified with diethylaminoethyl (DEAE) groups to create an anion exchanger, DEAE-SPAG for protein adsorption. Both optical microscopy and SEM revealed the superporous pellicular structure of the SPAG composite particle. With the average size (127.8 μm) and the mean density (1.7 g/ml), the DEAE-SPAG showed favorable an adsorption capacity of 148 mg/ml and a diffusivity of Dp, 2.6 × 10−11 m2/s for bovine serum albumin (BSA). All the results indicated that the SPAG medium is promising for effective protein adsorption.

Keywords
Superporous pellicular; Composite particle; Protein; Adsorption; Diffusion; Mass transfer
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Superporous pellicular agarose–glass composite particle for protein adsorption
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 34, Issue 2, May 2007, Pages 99–106
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us