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A dye–ligand immobilized poly(2-hydroxyethylmethacrylate) membrane used for adsorption and isolation of immunoglobulin G

Paper ID Volume ID Publish Year Pages File Format Full-Text
4909 256 2007 9 PDF Available
Title
A dye–ligand immobilized poly(2-hydroxyethylmethacrylate) membrane used for adsorption and isolation of immunoglobulin G
Abstract

Poly(2-hydroxyethylmethacrylate) (pHEMA) membrane was prepared by photo-polymerization and a dye ligand (i.e., Reactive Green 5) was immobilised on the membrane surface (pHEMA-RG-5). Surface wettability and hydrophilicity of the dye–ligand immobilised membranes were investigated by static contact angle measurements. The chromatographic properties of the dye–ligand immobilized membrane for adsorption and purification of IgG are discussed using bare membrane as a control system. The non-specific adsorption of IgG was low (0.45 mg protein ml−1 membrane). The attachment of dye ligand onto the membrane significantly increased the IgG adsorption (33.75 mg protein ml−1 membrane). The maximum IgG adsorption was observed at pH 6.0. The reversible IgG adsorption on the membrane obeyed both the Freundlich and the Langmuir–Freundlich isotherm models. To test the efficiency of IgG isolation from human serum with the pHEMA-RG-5 membrane, the purity of the eluted IgG, as determined by HPLC, was 81% with a recovery of 67%.

Keywords
Adsorption; Biomimetics; Microporous membranes; Separation; IgG; Contact angle
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A dye–ligand immobilized poly(2-hydroxyethylmethacrylate) membrane used for adsorption and isolation of immunoglobulin G
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 34, Issue 2, May 2007, Pages 147–155
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us