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Effects of shear stress and mass transfer on chitinase production by Paenibacillus sp. CHE-N1

Paper ID Volume ID Publish Year Pages File Format Full-Text
4912 256 2007 7 PDF Available
Title
Effects of shear stress and mass transfer on chitinase production by Paenibacillus sp. CHE-N1
Abstract

Chitinase fermentations with strain Paenibacillus sp. CHE-N1 was carried out in a 5-l stirred-tank bioreactor. The effects of operation variables including agitation rates, aeration rates, and pH values, on cell growth and chitinase activity yield were evaluated. Oxygen transfer limitation was predominant when the fermentation was carried out at low agitation rate (100 rpm). At agitation rate of 300 rpm, the high shear stress was dominant. Decrease chitinase production and extensive sporulation along with cell degradation were observed. When the pH was controlled at a constant (6.0–9.0) during the cultivation, lower chitinase production was obtained. The operation conditions at an aeration rate of 3 vvm, an agitation rate of 200 rpm, and without pH control could yield an optimal chitinase activity level of 11.8 U/ml.

Keywords
Chitinase; Stirred-tank bioreactor; Paenibacillus sp.; Shear stress; Mass transfer
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 34, Issue 2, May 2007, Pages 172–178
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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Full-text PDF Download
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Any Questions? feel free to contact us