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Optimization of solid-state medium for the production of inulinase by Kluyveromyces S120 using response surface methodology

Paper ID Volume ID Publish Year Pages File Format Full-Text
4913 256 2007 6 PDF Available
Title
Optimization of solid-state medium for the production of inulinase by Kluyveromyces S120 using response surface methodology
Abstract

The optimization of nutrient levels for the production of inulinase by a newly strain Kluyveromyces S120 in solid-state fermentation (SSF) was carried out using response surface methodology (RSM) based on Plackett–Burman design and Box–Behnken design. In the first optimization step, a Plackett–Burman design was used to evaluate the influence of related factors. Inulin, corn steep liquor and (NH4)2SO4 were found to be more compatible supplement with the substrate of wheat bran and positively influenced on inulinase production. In the second step, the concentrations of the three nutrients above were further optimized using a Box–Behnken design. The final concentration of medium optimized with RSM was 12.72% inulin, 10.76% corn steep liquor and 1.61% (NH4)2SO4 by employing wheat bran as the solid substrate. The average inulinase activity (409.8 U/g initial dry substrate) in triplicate under optimal medium was obtained.

Keywords
Response surface methodology; Optimization; Kluyveromyces S120; Inulinase production; Solid-state fermentation
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Optimization of solid-state medium for the production of inulinase by Kluyveromyces S120 using response surface methodology
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 34, Issue 2, May 2007, Pages 179–184
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us