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Alkaline protease production by submerged fermentation in stirred tank reactor using Bacillus licheniformis NCIM-2042: Effect of aeration and agitation regimes

Paper ID Volume ID Publish Year Pages File Format Full-Text
4914 256 2007 8 PDF Available
Title
Alkaline protease production by submerged fermentation in stirred tank reactor using Bacillus licheniformis NCIM-2042: Effect of aeration and agitation regimes
Abstract

The effects of aeration and agitation on alkaline protease production and protease yield were studied by submerged fermentation in a batch STR using Bacillus licheniformis NCIM-2042. The agitation rate varied in the range of 200–400 rpm at each airflow rates of 1–3 vvm. The maximum protease production was found on third day (72 h) and then decreased during fourth and fifth day of operation in all batches of STR operation. The best combination of airflow and agitation rate for the present system was at 3 vvm of airflow rate and 200 rpm of agitation rate on the basis of maximum specific protease production rate. Maximum specific protease production of 102 U/mg DC was observed on third day at 3 vvm and 200 rpm. The effects of casein concentrations on protease production, viscosity changes and oxygen transfer rate were studied. The increase in casein concentration has resulted in increased viscosity of the fermentation broth with increase in time of bioreactor operation, which in turn resulted in decreased oxygen mass transfer coefficient with reduced oxygen transfer rates.

Keywords
STR, stirred tank reactor; rpm, revolution per minute; vvm, volume of air per volume of media per minute; DO, dissolved oxygen; OTR, oxygen transfer rate; DCW, dry cell weightStirred tank reactor; Protease production; Yield; Viscosity; Volumetric oxygen m
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Alkaline protease production by submerged fermentation in stirred tank reactor using Bacillus licheniformis NCIM-2042: Effect of aeration and agitation regimes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 34, Issue 2, May 2007, Pages 185–192
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us