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Preparation of (S)-mandelic acid from racemate using growing cells of Pseudomonas putida ECU1009 with (R)-mandelate degradation activity

Paper ID Volume ID Publish Year Pages File Format Full-Text
4917 258 2006 5 PDF Available
Title
Preparation of (S)-mandelic acid from racemate using growing cells of Pseudomonas putida ECU1009 with (R)-mandelate degradation activity
Abstract

(S)-Mandelic acid [(S)-MA] was prepared in a one-pot fermentation-transformation process by a (R)-MA-degrading bacterium, Pseudomonas putida ECU1009. Using a sequential simplex optimization method, the best results were obtained with growing cells cultivated on a medium composed of 1.0% (RS)-MA, 0.1% NH4NO3, and 0.1% corn steep liquor. The optimal cultivation conditions were 30 °C and pH 6.5. By fed-batch culture under the optimal conditions, (S)-MA was successfully prepared in 1-L Erlenmeyer flask with 41% isolation yield and >99.9% ee, from totally 3% (RS)-MA after 94 h of biotransformation.

Keywords
(S)-Mandelic acid; Pseudomonas putida; Biodegradation; Fed-batch culture; Process optimization
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Preparation of (S)-mandelic acid from racemate using growing cells of Pseudomonas putida ECU1009 with (R)-mandelate degradation activity
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 30, Issue 1, 1 May 2006, Pages 11–15
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
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