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Supramolecular-mediated thermostabilization of phenylalanine dehydrogenase modified with β-cyclodextrin derivatives

Paper ID Volume ID Publish Year Pages File Format Full-Text
4919 258 2006 7 PDF Available
Title
Supramolecular-mediated thermostabilization of phenylalanine dehydrogenase modified with β-cyclodextrin derivatives
Abstract

Two different monoactivated β-cyclodextrin derivatives, named mono-6-amino-6-deoxy-β-CD (CD1) and mono-6-(5-carboxypentane-1-carboxamidoyl)-6-deoxy-β-CD (CD2) were evaluated as modifying agents for Bacillus badius phenylalanine dehydrogenase. The enzyme glycosidated with CD1 and CD2 contained about 18 mol and 15 mol oligosaccharide per mol of protein and retained 60% and 81% of the initial activity, respectively. The optimum temperature for the catalytic activity of phenylalanine dehydrogenase was increased in 10 °C after attaching the CDs residues. The enzyme thermostability profile was improved, and its resistance to thermal inactivation at different temperatures ranging from 45 °C to 60 °C was noticeably increased after glycosidation. The activation free energy of thermal inactivation was increased by 16.8 kJ/mol and 12.6 kJ/mol for the enzyme modified with CD1 and CD2, respectively. The influence of supramolecular host–guest associations on the improved thermotolerance showed by the modified enzyme forms was demonstrated by fluorescence spectroscopy and enzymatic measurements.

Keywords
Phenylalanine dehydrogenase; Protein denaturation; Enzyme technology; Amino acids; Enzyme activity; Cyclodextrin
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Supramolecular-mediated thermostabilization of phenylalanine dehydrogenase modified with β-cyclodextrin derivatives
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 30, Issue 1, 1 May 2006, Pages 26–32
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us