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Synthesis of structured phosphatidylcholine containing punicic acid by the lipase-catalyzed transesterification with pomegranate seed oil

Paper ID Volume ID Publish Year Pages File Format Full-Text
49218 46724 2016 5 PDF Available
Title
Synthesis of structured phosphatidylcholine containing punicic acid by the lipase-catalyzed transesterification with pomegranate seed oil
Abstract

•Egg-yolk phosphatidylcholine (PC) was enzymatically enriched with punicic acid.•Pomegranate seed oil was applied as the acyl donor.•Among tested enzymes lipase B from Candida antarctica was the most effective.•Optimal reaction parameters were chosen (oil:PC 3:1 M ratio and 20% enzyme load).•56% of punicic acid was incorporated into the sn-1 position of PC.

Incorporation of conjugated linolenic acid (punicic acid; 18:3, 9c,11t,13c) into egg-yolk phosphatidylcholine (PC) in the lipase-catalyzed transesterification process was the aim of this work. Pomegranate seed oil (PSO) containing over 77% of punicic acid was used as an acyl donor and three commercially available immobilized lipases were examined as biocatalysts. The effects of enzyme load, reaction time and molar ratio of substrates (PC:PSO) on the incorporation of punicic acid into the modified PC were tested. In all experiments the best results were obtained using lipase B from Candida antarctica. In optimum conditions (20% of enzyme load; 1:3 PC:PSO molar ratio; 72 h), the incorporation of punicic acid into the sn-1 position of PC was 56%. Additionally, the total content of polyunsaturated fatty acids in modified PC was almost 50%.

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Keywords
PC, phosphatidylcholine; PSO, pomegranate seed oil; CFA, conjugated fatty acid; PUFA, polyunsaturated fatty acid; CLA, conjugated linoleic acid; CLNA, conjugated linolenic acid; PL, phospholipids; ALA, α-linolenic acid; LPC, lysophosphatidylcholine; TAG,
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Synthesis of structured phosphatidylcholine containing punicic acid by the lipase-catalyzed transesterification with pomegranate seed oil
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Publisher
Database: Elsevier - ScienceDirect
Journal: Catalysis Communications - Volume 75, 5 February 2016, Pages 60–64
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us