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Purification and characterization of a bifunctional enzyme with chitosanase and cellulase activity from commercial cellulase

Paper ID Volume ID Publish Year Pages File Format Full-Text
4926 258 2006 6 PDF Available
Title
Purification and characterization of a bifunctional enzyme with chitosanase and cellulase activity from commercial cellulase
Abstract

A bifunctional enzyme with chitosanase and carboxymethyl cellulase (CMCase) activity was purified from commercial cellulase, which was produced by trichoderma viride, through sequential steps of DEAE-Sepharose CL-6B ion-exchange chromatography, Phenyl Sepharose CL-4B hydrophobic interaction chromatography and Sephadxe G-75 gel filtration. The purified hydrolase was homogeneous as examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular mass was 66 kDa. The hydrolase exhibited chitosanase activity for chitosan hydrolysis and cellulase activity for carboxymethyl cellulose (CMC) hydrolysis. For chitosan hydrolysis, the enzyme had an optimum pH of 5.2, temperature of 60 °C and exhibited typical Michaelis-Menten kinetics with Km value and Vmax of 10 mg/ml and 0.164 U/ml, respectively. For CMC hydrolysis, the pH and temperature optima enzyme were 4.0 and 50 °C. Heavy metal ions such as Hg2+, Ag+ significantly or completely inhibited the enzyme activity. Identification of glucosamine (GlcN) and N-acetyl-glucosamine (GlcNAc) oligomers as depolymerized products indicated that the enzyme cleaved both GlcN-GlcN and GlcNAc-GlcN linkages. The chitosan hydrolysates were oligomers with one to four glucosamine residues and some oligomers with longer chain length.

Keywords
Cellulase; Chitosanase; Bifunctional; Properties; trichoderma viride
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Purification and characterization of a bifunctional enzyme with chitosanase and cellulase activity from commercial cellulase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 30, Issue 1, 1 May 2006, Pages 82–87
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us