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Partitioning of β-glucosidase from Trichoderma reesei in poly(ethylene glycol) and potassium phosphate aqueous two-phase systems: Influence of pH and temperature

Paper ID Volume ID Publish Year Pages File Format Full-Text
4929 258 2006 5 PDF Available
Title
Partitioning of β-glucosidase from Trichoderma reesei in poly(ethylene glycol) and potassium phosphate aqueous two-phase systems: Influence of pH and temperature
Abstract

This study evaluates the influence of pH and temperature on the partition coefficient of β-glucosidase and total protein in order to determine the best conditions for the purification of the enzyme in poly(ethylene glycol) (PEG) 4000 (8%, w/w) and potassium phosphate salt (13%, w/w) aqueous two-phase systems. The partition coefficient increased with pH and temperature for the ranges investigated (i.e., pH: 6.0–7.5; temperature: 25–55 °C). Yield and selectivity were key criteria for the selection of the optimum separation condition for enzyme purification. The highest selectivity was achieved at a pH of 6.5 and a temperature of 25 °C. A β-glucosidase yield greater than 92% was obtained in the bottom phase for pH values less than 7.0 and temperatures below 35 °C.

Keywords
Aqueous two-phase system; Bioseparations; β-d-Glucosidase; Protein; Purification; Partition coefficient
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Partitioning of β-glucosidase from Trichoderma reesei in poly(ethylene glycol) and potassium phosphate aqueous two-phase systems: Influence of pH and temperature
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 30, Issue 1, 1 May 2006, Pages 104–108
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us