fulltext.study @t Gmail

Development of optimal culture method of Sparassis crispa mycelia and a new extraction method of antineoplastic constituent

Paper ID Volume ID Publish Year Pages File Format Full-Text
4930 258 2006 5 PDF Available
Title
Development of optimal culture method of Sparassis crispa mycelia and a new extraction method of antineoplastic constituent
Abstract

The optimal culture condition of basidiomycete Sparassis crispa mycelia was determined for producing a large amount of antineoplastic constituent, i.e. β-1,3-glucan. Furthermore, a new extraction method with steam explosion as a pretreatment was attempted to increase the amount of antineoplastic constituent extracted from S. crispa mycelia. The shake-flask liquid culture of fungal mycelia could shorten a culture time up to about 1/3 compared with the conventional stationary liquid culture. The productivity of S. crispa mycelia reached its maximum at an initial glucose concentration of 30 g/L, a culture temperature of 25–30 °C, and pH 5. Steam explosion at a steam temperature of 225 °C for 5 min not only enhanced the amount of antineoplastic constituent extracted from the mycelia but also shortened the extraction time significantly.

Keywords
Bioconversion; Filamentous fungi; β-1,3-Glucan; Microbial growth; Plant cell culture; Sparassis crispa
First Page Preview
Development of optimal culture method of Sparassis crispa mycelia and a new extraction method of antineoplastic constituent
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 30, Issue 1, 1 May 2006, Pages 109–113
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering