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Immobilization of Aspergillus niger xylanase A on Fe3O4-coated chitosan magnetic nanoparticles for xylooligosaccharide preparation

Paper ID Volume ID Publish Year Pages File Format Full-Text
49487 46750 2014 5 PDF Available
Title
Immobilization of Aspergillus niger xylanase A on Fe3O4-coated chitosan magnetic nanoparticles for xylooligosaccharide preparation
Abstract

•Fe3O4-coated chitosan was prepared by the LBL self-assembly approach.•XylA was immobilized on Fe3O4-coated chitosan via covalent bond.•Immobilized XylA can be recycled easily and exhibited improved stability.•Immobilized XylA released high concentration of XOs from birchwood xylan.

Aspergillus niger xylanase A (XylA) was immobilized onto Fe3O4-coated chitosan magnetic nanoparticles prepared by the layer-by-layer self-assembly approach. The Fe3O4-coated chitosan magnetic nanoparticles showed a high binding capacity of 162.2 mg ∙ g− 1-particles and a recovery activity of 56.5% for XylA. The immobilized XylA showed improved thermostability and storage stability compared with free XylA. The immobilized XylA retained 87.5% activity after seven successive reactions by magnetic separation. Xylotriose and xylohexaose were the main products released from birchwood xylan and wheat bran insoluble xylan by immobilized XylA, respectively.

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Keywords
Xylanase; Immobilization; Magnetic nanoparticles; Xylooligosaccharides; Layer-by-layer (LBL)
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Immobilization of Aspergillus niger xylanase A on Fe3O4-coated chitosan magnetic nanoparticles for xylooligosaccharide preparation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Catalysis Communications - Volume 55, 5 October 2014, Pages 6–10
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
Get Full-Text Now
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Price was $35.95
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Price after discount Only $4.95
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Full-text PDF Download
Online Support
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