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Production of laccase by membrane-surface liquid culture of Trametes versicolor using a poly(l-lactic acid) membrane

Paper ID Volume ID Publish Year Pages File Format Full-Text
4956 261 2007 4 PDF Available
Title
Production of laccase by membrane-surface liquid culture of Trametes versicolor using a poly(l-lactic acid) membrane
Abstract

Membrane-surface liquid culture (MSLC) is a promising method for the bioproduction of highly aerobic filamentous fungi [A. Ogawa, A. Yasuhara, T. Tanaka, T. Sakiyama, K. Nakanishi, Production of neutral protease by membrane-surface liquid culture of Aspergillus oryzae IAM2704, J. Ferment. Bioeng. 80 (1995) 35–40]. This paper reports on the production of laccase by Trametes versicolor on a microporous membrane of poly(l-lactic acid) (PLLA), which can be biodegraded via composting after use. The membrane was stable as a support for 24 days at 30 °C. During the first 9 days in MSLC, the fungus produced half as much laccase as it did in liquid-surface culture (LSC); however, the mycelium on the membrane was able to be re-used five times for laccase production. The laccase production was accelerated in the repeated use of the culture while the mycelium in LSC ceased to produce the enzyme. This study shows that compostable PLLA microporous membranes can be used for enzyme production by MSLC of filamentous fungi.

Keywords
Membrane bioreactors; Filamentous fungi; Microporous membranes; Poly(l-lactic acid); Enzyme production; Laccase
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Production of laccase by membrane-surface liquid culture of Trametes versicolor using a poly(l-lactic acid) membrane
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 33, Issue 2, February 2007, Pages 188–191
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us