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Mathematical model for stepwise hydrolysis of triolein using Candida rugosa lipase in biphasic oil–water system

Paper ID Volume ID Publish Year Pages File Format Full-Text
4962 264 2006 8 PDF Available
Title
Mathematical model for stepwise hydrolysis of triolein using Candida rugosa lipase in biphasic oil–water system
Abstract

A mathematical model describing the triglyceride hydrolysis with lipase was constructed. The stepwise hydrolysis at the oil–water interface based on the Ping Pong Bi Bi mechanism and the inhibition by a fatty acid were considered in addition to the differences in the interfacial and bulk concentrations of the enzyme, substrate and product. This model was applied to the experimental results for the triolein hydrolysis using Candida rugosa lipase in the biphasic oil–water system under various operating conditions. In order to discuss the model's advantages, other simple models not considering the inhibition by a fatty acid or Ping Pong Bi Bi mechanism were also applied to our experimental results. The present model gave better fitted results and well described the effect of the initial enzyme concentration, the interfacial area and the initial concentrations of triolein and a fatty acid on the entire process of the stepwise triolein hydrolysis. The present model was very effective for predicting the appropriate conditions for the efficient production of the desired product and to provide information which cannot be experimentally obtained.

Keywords
Biphasic oil–water system; Inhibition by fatty acid; Lipase; Modelling; Ping Pong Bi Bi mechanism; Triglyceride hydrolysis
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Mathematical model for stepwise hydrolysis of triolein using Candida rugosa lipase in biphasic oil–water system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 31, Issue 2, 1 September 2006, Pages 125–132
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us