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Synergistic effect of co-expressing d-amino acid oxidase with T7 lysozyme on self-disruption of Escherichia coli cell

Paper ID Volume ID Publish Year Pages File Format Full-Text
4972 265 2006 6 PDF Available
Title
Synergistic effect of co-expressing d-amino acid oxidase with T7 lysozyme on self-disruption of Escherichia coli cell
Abstract

Recombinant Escherichia coli with self-disruption characteristic was developed. The cell harbored a d-amino acid oxidase (DAAO) expressing plasmid resulted in an obvious self-disruption. When T7 lysozyme instead of DAAO was used as a lysis gene, no appreciable self-disruption was observed. In the presence of two compatible plasmids, which expressed DAAO and T7 lysozyme, respectively, E. coli BL21(DE3) showed a significant self-disruption. A lysis plasmid containing both inducible DAAO and constitutive T7 lysozyme genes were, therefore, constructed to make the transformed cells self-disrupt more effectively. Green fluorescent protein (GFP), a model target protein, was co-expressed in this self-disruptive E. coli cell. About 60% of the cells were self-disrupted when the lysis genes were induced by isopropyl thiogalactoside (IPTG). The cell disruption efficiency reached to 90% after freeze/thawing treatment.

Keywords
Escherichia coli; Self-disruption; d-amino acid oxidase; T7 lysozyme; Green fluorescent protein
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Synergistic effect of co-expressing d-amino acid oxidase with T7 lysozyme on self-disruption of Escherichia coli cell
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 28, Issue 1, February 2006, Pages 17–22
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us