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Efficient production of recombinant protein in immobilized insect cell culture using serum-free basal media after baculovirus infection

Paper ID Volume ID Publish Year Pages File Format Full-Text
4979 265 2006 6 PDF Available
Title
Efficient production of recombinant protein in immobilized insect cell culture using serum-free basal media after baculovirus infection
Abstract

To develop an efficient biological production process using the baculovirus–insect cell system, recombinant protein production was investigated in an immobilized cell culture with medium replacement. Sf9 insect cells were naturally entrapped within reticulated polyvinyl formal resin biomass support particles (BSPs; 2 mm × 2 mm × 2 mm; pore diameter 30–50 μm) in a 2.5-l stirred-tank bioreactor. The immobilized cells were grown to a density of over 107 cells/cm3-BSP, with the medium of 10% fetal bovine serum (FBS)-supplemented TNM-FH replaced at appropriate intervals, before infection with a recombinant baculovirus carrying the β-galactosidase gene. When serum-free TNM-FH was used instead of 10% FBS-supplemented TNM-FH for medium replenishment on and after post-infection day 1, the immobilized cells showed a high β-galactosidase yield comparable to that obtained in a culture using the serum-supplemented medium throughout. This finding indicates that immobilized insect cell culture allows not only intensification of cell culture but also recombinant protein production in protein-free basal media.

Keywords
Insect cell culture; Baculovirus; Immobilization; Biomass support particles; Bioreactors; Recombinant protein production
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Efficient production of recombinant protein in immobilized insect cell culture using serum-free basal media after baculovirus infection
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 28, Issue 1, February 2006, Pages 67–72
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us