fulltext.study @t Gmail

Microbial production of 1,3-propanediol from glycerol by encapsulated Klebsiella pneumoniae

Paper ID Volume ID Publish Year Pages File Format Full-Text
4992 266 2006 7 PDF Available
Title
Microbial production of 1,3-propanediol from glycerol by encapsulated Klebsiella pneumoniae
Abstract

Klebsiella pneumoniae was encapsulated in the NaCS/PDMDAAC (sodium cellulose sulfate/poly-dimethyl-diallyl-ammonium chloride) microcapsule to produce 1,3-propanediol (PDO) from glycerol. Fermentations including batch culture, fed-batch culture and continuous culture were carried out in shake flasks and a fixed-bed bioreactor. Results showed that the NaCS/PDMDAAC microcapsule has desirable properties – such as biomass enrichment in the microcapsule (6.84 g/L capsule, 2.6-fold higher than that of free cell culture), enhanced substrate tolerance and stable activity of cells, operation convenience for continuous culture – for the microbial production of 1,3-propanediol. A 1,3-propanediol concentration of 63.1 g/L with a yield of 0.65 mol PDO/mol glycerol was obtained in the batch culture. The encapsulated cells remained its activity for 67 h in the fed-batch culture and produced 1,3-propanediol at a concentration of 51.86 g/L. In the continuous culture, 1,3-propanediol was provided at a productivity of 4.49 g/L h, a concentration of 13.6 g/L, and a yield of 0.43 mol PDO/mol glycerol at a dilution rate of 0.33 h−1.

Keywords
1,3-Propanediol; Bioconversion; Immobilized cells; NaCS/PDMDAAC (sodium cellulose sulfate/poly-dimethyl-diallyl-ammonium chloride) microcapsules; Fixed-bed bioreactor; Glycerol
First Page Preview
Microbial production of 1,3-propanediol from glycerol by encapsulated Klebsiella pneumoniae
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 32, Issue 2, 15 November 2006, Pages 93–99
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us