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Production and partial characterization of endoxylanase by Bacillus pumilus using agro industrial residues

Paper ID Volume ID Publish Year Pages File Format Full-Text
4994 266 2006 7 PDF Available
Title
Production and partial characterization of endoxylanase by Bacillus pumilus using agro industrial residues
Abstract

Agro industrial residues, cheap sources of energy have high potential in the area of fermentation for the production of lignocellulases. Different agro industrial waste products were evaluated as substrates in submerged and solid-state fermentation for xylanolytic enzyme production by Bacillus pumilus and the enzyme titers were compared. Higher titer of endoxylanase was obtained with solid state than with liquid fermentation with feeble amount of cellulase. High proteolytic activity was observed in submerged fermentation where as in solid state the proteolytic activity was lower in all cases except in media supplemented with soy meal due to its high protein content. The endoxylanase produced by solid-state fermentation was stable over a wide range of pH and temperatures. The highest enzyme activity was obtained at pH 6.5–7 and at 50 °C. The enzyme could retain 30% of its activity even after 1 h at 60 °C. Considering the conditions under which Kraft pulps are bleached during the manufacture of paper, xylanase from B. pumilus exhibit favorable potential for application in the paper processing industries.

Keywords
Submerged fermentation; Solid-state fermentation; Bacillus pumilus; Endoxylanase; Cellulase; Proteases
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Production and partial characterization of endoxylanase by Bacillus pumilus using agro industrial residues
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 32, Issue 2, 15 November 2006, Pages 106–112
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us