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Direct recovery of clotting factor IX from unclarified human plasma by expanded bed ion exchange chromatography

Paper ID Volume ID Publish Year Pages File Format Full-Text
5002 269 2006 9 PDF Available
Title
Direct recovery of clotting factor IX from unclarified human plasma by expanded bed ion exchange chromatography
Abstract

In this study, STREAMLINE DEAE adsorbent was used for the direct recovery of clotting factor IX (FIX) from unclarified human plasma in expanded bed adsorption (EBA). The equilibrium adsorption studies for general protein showed that the experimental data followed the Langmuir isotherm model. The maximum adsorption capacity for general protein was 35.33 mg/ml with a dissociation constant of 0.18 mg/ml. However, the adsorption of FIX followed the Henry's law with Henry's constant of 59.78. Optimal condition for the elution of FIX with step changes of increasing concentration of NaCl was determined in small packed bed experiments conducted with clarified plasma. The results showed 94.28% of the bound FIX could be eluted with 0.7 M NaCl. The influence of liquid velocities of 100–300 cm/h on the selective adsorption of FIX from clarified plasma was also investigated in packed bed experiments. The results showed that the rate of FIX breakthrough was found to be very similar. In this work, operation at a constant liquid velocity of 200 cm/h in an expanded bed recovery process was chosen for the purification of FIX. The FIX was directly recovered using 0.7 M NaCl from unclarified plasma with a purification factor of 14 and a yield of 90% in a single step.

Keywords
APTT, activated partial thromboplastin time; DBC, dynamic binding capacity; DEAE, dimethylaminoethyl group; DF, dilution factor; E1–2, elution steps 1–2 for FIX; EBA, expanded bed adsorption; EBIEC, expanded bed ion exchange chromatography; F + W, flow th
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Direct recovery of clotting factor IX from unclarified human plasma by expanded bed ion exchange chromatography
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 30, Issue 2, 1 June 2006, Pages 138–146
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us