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High-density culture of hepatocytes in a packed-bed bioreactor using a fibrous scaffold from plant

Paper ID Volume ID Publish Year Pages File Format Full-Text
5009 269 2006 7 PDF Available
Title
High-density culture of hepatocytes in a packed-bed bioreactor using a fibrous scaffold from plant
Abstract

A vegetable sponge (loofa sponge, LS) from the fibrous interior of dried fruits (Luffa cylindrica) was used as a scaffold in a packed-bed bioreactor for dynamic seeding and perfusion culture of rat hepatocytes. Hepatocytes were immobilized within the highly complex macroscopic architecture of LS by attaching to its rough fiber surface. The cell seeding efficiency was independent of the initial loading cell number up to 2 × 108 hepatocytes (1 × 107 cells/cm3 scaffold) and cell distribution within the bioreactor was uniform. Liver-specific functions of the hepatocytes depended on cell seeding density and medium flow rate with the optimum cell density at 3.5 × 106 cells/cm3 scaffold. Spheroids with diameters around 100 μm and high viability from FDA staining were observed at day 3 and spheroids as large as 400 μm could be found at day 4. Cell damage was enhanced due to direct contacting of cells with the flow stream. Nonetheless, hepatocytes metabolic rates were substantially improved over stationary culture on TCPS dishes with production rates of albumin and urea at day 7 maintained at 90% and 67% of their initial values, respectively.

Keywords
Hepatocytes; Bioartificial liver; Immobilized cells; Tissue cell culture; Bioreactors; Biomedical
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High-density culture of hepatocytes in a packed-bed bioreactor using a fibrous scaffold from plant
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 30, Issue 2, 1 June 2006, Pages 192–198
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us