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Increased total air pressure versus oxygen limitation for enhanced oxygen transfer and product formation in a Pichia pastoris recombinant protein process

Paper ID Volume ID Publish Year Pages File Format Full-Text
5011 269 2006 7 PDF Available
Title
Increased total air pressure versus oxygen limitation for enhanced oxygen transfer and product formation in a Pichia pastoris recombinant protein process
Abstract

Two strategies to increase the productivity of secreted Thai Rosewood β-glucosidase in Pichia pastoris processes were evaluated. Both methods were based on increasing the oxygen transfer rate (OTR) in the process by simple means. Increasing the driving force for the diffusion from the air bubbles to the medium by elevating the air pressure, from 1.2 to 1.9 bar increased the oxygen uptake rate (OUR) by 59% while increasing the driving force by accepting oxygen limitation increased the OUR by 35%. The OTR increased less than in proportion to the increased solubility in the high-pressure process, which indicates that air bubble compression reduces the volumetric oxygen transfer coefficient (KLa). Even though the methanol consumption increased almost in proportion to the OTR in both processes the biomass production did not increase as much. This is explained as a higher maintenance demand for methanol in the oxygen limited (0.027 g g−1 g−1) and high-pressure processes (0.035 g g−1 g−1), compared to 0.022 g g−1 g−1 in the methanol limited reference process. However, in spite of the low effect of increasing OTR on the biomass production the total β-glucosidase yield increased almost in proportion to the increased methanol consumption and reached highest value in the high-pressure process, while the β-glucosidase purity was highest in the oxygen-limited process due to release of less contaminating proteins.

Keywords
Aeration; Oxygen transfer; Dissolved oxygen tension; Enzyme production; β-Glucosidase; Oxygen-limited fed-batch
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Increased total air pressure versus oxygen limitation for enhanced oxygen transfer and product formation in a Pichia pastoris recombinant protein process
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 30, Issue 2, 1 June 2006, Pages 205–211
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us