Insight into enzymatic synthesis of phosphatidylserine in deep eutectic solvents
•Enzymatic phosphatidylserine synthesis in deep eutectic solvents was developed.•> 90% yield of phosphatidylserine could be achieved.•81% original activity of enzyme was maintained after being used for 10 batches.
Deep eutectic solvents (DESs) have recently attracted a significant interest in numerous fields including biocatalysis because they are inexpensive, environmentally friendly, non-toxic, biodegradable, and enzyme compatible. Herein, these solvents were successfully used as the reaction media for enzyme-mediated transphosphatidylation of phosphatidylcholine with l-serine for the synthesis of phosphatidylserine for the first time. Enzymatic phosphatidylserine synthesis in various DESs was comprehensively investigated, employing phospholipase D. Our results indicated that > 90% yield of phosphatidylserine could be achieved using choline chloride/ethylene glycol as DES. Furthermore, 81% original activity of the enzyme was maintained after being used for 10 batches. This study indicates that DESs act as potential candidates for the eco-friendly solvents in biocatalysis applications.
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Journal: Catalysis Communications - Volume 82, 5 July 2016, Pages 16–19