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Optimization of culture conditions for CO2 fixation by a chemoautotrophic microorganism, strain YN-1 using factorial design

Paper ID Volume ID Publish Year Pages File Format Full-Text
5017 271 2006 7 PDF Available
Title
Optimization of culture conditions for CO2 fixation by a chemoautotrophic microorganism, strain YN-1 using factorial design
Abstract

Culture conditions for CO2 fixation by a chemoautotrophic microorganism, strain YN-1, were optimized by statistical analysis using factorial design. Statistical analysis was performed by SAS software and then predicted the levels of four factors, H2 (X1), O2 (X2), CO2 (X3) concentration and pH (X4) which were required to obtain the optimum culture conditions. For the fractional factorial design 34−1, total 27 experimental runs by combination of each factor were carried out in a 5 L-jar fermentor, and the three-dimensional response surfaces were plotted to find out the optimum level of each factor for maximum CO2 fixation. Dry cell weight in optimized culture condition was predicted as 11.4 g/L in 48 h under the condition of 37.5 × 10−3 mol/min H2, 9.38 × 10−3 mol/min O2, 4.91 × 10−3 mol/min CO2, and pH 6.7. As compared with CO2 fixation rate before optimizing culture conditions, the improvement of CO2 fixation rate under optimized culture conditions was experimentally confirmed.

Keywords
Chemoautotrophic microorganism; CO2 fixation; Dry cell weight; Factorial design; SAS software
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Optimization of culture conditions for CO2 fixation by a chemoautotrophic microorganism, strain YN-1 using factorial design
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 31, Issue 1, 1 August 2006, Pages 1–7
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us