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Improved cavitational cell disruption following pH pretreatment for the extraction of β-galactosidase from Kluveromyces lactis

Paper ID Volume ID Publish Year Pages File Format Full-Text
5020 271 2006 6 PDF Available
Title
Improved cavitational cell disruption following pH pretreatment for the extraction of β-galactosidase from Kluveromyces lactis
Abstract

The efficient release of intracellular β-galactosidase by ultrasonic disruption of cells treated with aqueous solutions of different pH has been investigated to improve the cavitational efficacy of the cell disruption process. Pretreatment of the cells at different pHs for various time intervals was studied. The maximum yield (95 ± 3 U/ml) was obtained by pretreatment of the cells at pH 4.4 for 6 h and subsequent ultrasonic cell disruption for 40 min. The maximum yield on ultrasonic disruption without pretreatment was 7.2 ± 0.6 U/ml. The energy efficiency of the disruption process for releasing β-galactosidase using different pre-treatments has been calculated and compared.

Keywords
Cell disruption; pH treatment; Cavitation; Enzyme release
First Page Preview
Improved cavitational cell disruption following pH pretreatment for the extraction of β-galactosidase from Kluveromyces lactis
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 31, Issue 1, 1 August 2006, Pages 25–30
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering