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Comparative study on the effects of n-dodecane addition on oxygen transfer in stirred bioreactors for simulated, bacterial and yeasts broths

Paper ID Volume ID Publish Year Pages File Format Full-Text
5024 271 2006 11 PDF Available
Title
Comparative study on the effects of n-dodecane addition on oxygen transfer in stirred bioreactors for simulated, bacterial and yeasts broths
Abstract

The efficiency of oxygen transfer into the fermentation broths could be enhanced by adding in broths oxygen-vectors, such as hydrocarbons or fluorocarbons, without increasing the energy consumption for mixing or aeration. The experimental results obtained for simulated, Propionibacterium shermanii and Saccharomyces cerevisiae broths indicated the considerable increase of kLa and (kLa)v/(kLa)0 by adding n-dodecane, but the magnitude of this effect must be correlated with broths and biomass characteristics, especially with the cells affinity for oxygen-vector droplets. Thus, due to the higher affinity of yeasts cells for hydrocarbon droplets, the increase of oxygen mass transfer rate was lower than that recorded for simulated or bacterial broths.By means of the experimental data, some mathematical correlations describing the influences of the main parameters (apparent viscosity, concentration of biomass, specific power input, superficial air velocity) on kLa in presence of n  -dodecane have been proposed for each considered fermentation systems. These equations have the general expression of kLa=α(Pa/V)βvsγηaδ or kLa=α(Pa/V)βvsγCxδ, the coefficients (β, γ and δ values being dependent on n-dodecane concentration, and offer a good agreement with the experiment, the average deviations being between ±9.6 and ±10.8%.

Keywords
Bioreactors; Oxygen transfer; Oxygen-vector; kLa; Simulated broths; Propionibacterium shermanii; Saccharomyces cerevisiae
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Comparative study on the effects of n-dodecane addition on oxygen transfer in stirred bioreactors for simulated, bacterial and yeasts broths
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 31, Issue 1, 1 August 2006, Pages 56–66
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us