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Strain improvement and optimization of the media of taxol-producing fungus Fusarium maire

Paper ID Volume ID Publish Year Pages File Format Full-Text
5025 271 2006 7 PDF Available
Title
Strain improvement and optimization of the media of taxol-producing fungus Fusarium maire
Abstract

A mutant Fusarium maire K178 with high production of paclitaxel (taxol) was selected by protoplast mutation of UV radiation and diethyl sulfate (DES). The optimization of medium components was performed using both Plackett–Burman design and response surface methodology. Various nitrogen sources were initially compared and the Plackett–Burman design was employed for screening important components in trace elements. The results showed that NaOAc, NH4NO3 and MgSO4 were the most important components which were further investigated by response surface methodology. The optimal concentrations were: NaOAc 2.02 g/l, NH4NO3 7.84 g/l and MgSO4 0.68 g/l. After the strain improvement and optimization of the media the yield of taxol increased from 20 to 225.2 μg/l.

Keywords
Taxol; Mutant; Response surface methodology; Plackett–Burman design; Fermentation; Protoplast
First Page Preview
Strain improvement and optimization of the media of taxol-producing fungus Fusarium maire
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 31, Issue 1, 1 August 2006, Pages 67–73
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering