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Changing enzymatic conformation in organic media with pH buffer lyophilized powder

Paper ID Volume ID Publish Year Pages File Format Full-Text
50276 46787 2015 4 PDF Available
Title
Changing enzymatic conformation in organic media with pH buffer lyophilized powder
Abstract

•Conformational memory of enzyme can be changed in organic media.•The conformational rigidity of enzyme in organic phase is relative.•The change may be influenced by the spatial redundancy of enzyme.

The conformational memory of enzyme has a significant application in biocatalysis. In this work, pH modification of tannase and the relevant factors were studied. This results indicate that direct addition of pH buffer lyophilized powder in organic media can remarkably change the conformation as did pH tunning, and the conformation variation closely correlates with the balance between hydrophilic solvents and hydrophobic solvents in reaction system. pH tunning of inner tannase and free tannase was performed. The results show that the variation may be affected by its spatial redundancy. It will be potentially applied in biocatalysis in organic media.

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Keywords
Biocatalysis; pH tunning; Conformational memory; Tannase; Bio-imprinting
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Changing enzymatic conformation in organic media with pH buffer lyophilized powder
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Publisher
Database: Elsevier - ScienceDirect
Journal: Catalysis Communications - Volume 65, 5 May 2015, Pages 62–65
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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