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Optimization of enzymatic hydrolysis of triglycerides in soy deodorized distillate with supercritical carbon dioxide

Paper ID Volume ID Publish Year Pages File Format Full-Text
5039 274 2006 7 PDF Available
Title
Optimization of enzymatic hydrolysis of triglycerides in soy deodorized distillate with supercritical carbon dioxide
Abstract

Enzymatic hydrolysis of triglycerides of soy deodorized distillate (DOD), using immobilized Candida rugosa lipase under supercritical carbon dioxide (SC-CO2) medium, was carried out. Optimization of the reaction parameters using response surface methodology based on Box-Behnken model at three levels of pressure (120–180 bar), temperature (40–60 °C) and moisture content (40–80% of triglyceride content) for maximum hydrolysis of triglycerides was arrived by multilinear regression of the experimental results. The optimum conditions for maximum degree of triglyceride hydrolysis (94%) were found to be: pressure of 180 bar, temperature of 43 °C and moisture content of 40% to the triglyceride content. Maximum degree of hydrolysis was achieved with short incubation time of 1.5 h under SC-CO2. Whereas conventional method of hydrolysis in hexane under similar reaction conditions of temperature, moisture and enzyme concentration, needs 5 h to achieve 88% of triglyceride hydrolysis.

Keywords
Soy deodorized distillate (DOD); Supercritical carbon dioxide (SC-CO2); Lipase; Immobilization; Zeolite; Optimization
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Optimization of enzymatic hydrolysis of triglycerides in soy deodorized distillate with supercritical carbon dioxide
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 29, Issue 3, 15 April 2006, Pages 220–226
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us