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A simple feedback control of Escherichia coli growth for recombinant aldolase production in fed-batch mode

Paper ID Volume ID Publish Year Pages File Format Full-Text
5041 274 2006 8 PDF Available
Title
A simple feedback control of Escherichia coli growth for recombinant aldolase production in fed-batch mode
Abstract

Fed-batch production of recombinant fuculose-1-phosphate aldolase (FucA) by Escherichia coli XL1 Blue MRF′ (pTrcfuc) has been automated by using a simple feedback specific growth rate control strategy. Non-induced continuous cultures were conducted in order to characterize substrate consumption and carbon dioxide production yields and rates. In fed-batch cultures, substrate feeding rate was adjusted using on-line biomass estimation based on exhaust gas analysis and macroscopic mass balances. Overexpression of recombinant protein induced by isopropyl-β-d-thiogalactopyranoside (IPTG) under trc promoter did not affect significantly the control of specific growth rate during 7 h after induction. Growth and protein production curves were parallel until high level of protein expression started to inhibit cell growth. The proposed specific growth rate control strategy has been successfully applied to both non-induced and induced fed-batch cultures that do not exhibit severe growth rate depression.

Keywords
Escherichia coli; Recombinant aldolases; Fed-batch culture; Enzyme production; Modelling; Feedback control
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A simple feedback control of Escherichia coli growth for recombinant aldolase production in fed-batch mode
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 29, Issue 3, 15 April 2006, Pages 235–242
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us