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Production of isomaltulose using Erwinia sp. D12 cells: Culture medium optimization and cell immobilization in alginate

Paper ID Volume ID Publish Year Pages File Format Full-Text
5046 274 2006 8 PDF Available
Title
Production of isomaltulose using Erwinia sp. D12 cells: Culture medium optimization and cell immobilization in alginate
Abstract

Isomaltulose is a structural isomer of sucrose commercially used in food industries. Glucosyltransferase produced by Erwinia sp. D12 catalyses an intramolecular transglucosylation of sucrose giving isomaltulose. An experimental Design and Response Surface Methodology were applied for the optimization of the nutrient concentration in the culture medium for enzyme production in shaken flasks at 200 rpm and 30 °C. A higher production of glucosyltransferase (7.47 Uml−1) was observed in the culture medium containing sugar cane molasses (160 gl−1), bacteriological peptone (20 gl−1) and yeast extract Prodex Lac SD® (15 gl−1) after 8 h, at 30 °C. The highest production of glucosyltransferase in the 6.6-l bioreactor (14.6 Uml−1) was obtained in the optimized culture medium after 10 h at 26 °C. When Erwinia sp. D12 cells were immobilized in sodium alginate, it was verified that sodium alginate solution A could be substituted by a cheaper one, sodium alginate solution B. Using a 40% cell suspension and 2% sodium alginate solution B for cell immobilization in a packed-bed reactor, 64.1% conversion of sucrose to isomaltulose was obtained. The packed-bed reactor with immobilized cells plus glutaraldehyde and polyethylenimine solutions remained in a pseudo-steady-state for 180 h.

Keywords
Experimental design; Immobilized cells; Isomaltulose; Optimization; Packed-bed reactor; Response surface methodology
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Production of isomaltulose using Erwinia sp. D12 cells: Culture medium optimization and cell immobilization in alginate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 29, Issue 3, 15 April 2006, Pages 270–277
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us