fulltext.study @t Gmail

Directed evolution of Candida antarctica lipase B for kinetic resolution of profen esters

Paper ID Volume ID Publish Year Pages File Format Full-Text
50495 46797 2013 5 PDF Available
Title
Directed evolution of Candida antarctica lipase B for kinetic resolution of profen esters
Abstract

•Candida antarctica lipase B was subjected to directed evolution by CAST and ISM.•Mutants showed increased activity and selectivity toward three profen esters.•High enantioselectivity for flurbiprofen and ketoprofen esters was obtained.

The lipase B from Candida antarctica was subjected to directed evolution suggested by its structure. Mutants of the lipase show significantly increased activity and enantioselectivity toward profen esters compared to the wild type, especially for flurbiprofen ester (ee = 93%, E = 37) and ketoprofen ester (ee = 99%, E > 200).

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Biocatalysis; Directed evolution; Lipases; Kinetic resolution; Profens
First Page Preview
Directed evolution of Candida antarctica lipase B for kinetic resolution of profen esters
Publisher
Database: Elsevier - ScienceDirect
Journal: Catalysis Communications - Volume 38, 5 August 2013, Pages 1–5
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis