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Efficient biocatalytic hydrolysis of 2-chloronicotinamide for production of 2-chloronicotinic acid by recombinant amidase

Paper ID Volume ID Publish Year Pages File Format Full-Text
50496 46797 2013 4 PDF Available
Title
Efficient biocatalytic hydrolysis of 2-chloronicotinamide for production of 2-chloronicotinic acid by recombinant amidase
Abstract

•A recombinant amidase was first successfully applied to the synthesis of 2-chloronicotinic acid.•Catalytic properties of recombinant amidase synthesizing 2-chloronicotinic acid were studied.•The recombinant amidase exhibited high activity and strong substrate concentration tolerant.

For efficient production of 2-chloronicotinic acid from 2-chloronicotinamide, an amidase gene was synthesized by codon-optimized and expressed. The optimum pH and temperature for the biocatalytic process were 7.5 and 45–50 °C, respectively. The recombinant E. coli whole cells exhibited tolerance against a high substrate concentration of up to 180 mM. It could also be repeatedly used in the biotransformation and the residual activity after 10 batches was still over 50% of the initial activity. These results indicated this recombinant amidase is of great potential for the practical production of 2-chloronicotinic acid.

Graphical abstractIn this study, a recombinant amidase from R. erythropolis was first successfully applied to the synthesis of 2-chloronicotinic acid under the mild conditions. The recombinant amidase exhibited stable, high activity and strong substrate concentration tolerant.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Recombinant amidase; 2-Chloronicotinic acid; 2-Chloronicotinamide; Biosynthesis
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Efficient biocatalytic hydrolysis of 2-chloronicotinamide for production of 2-chloronicotinic acid by recombinant amidase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Catalysis Communications - Volume 38, 5 August 2013, Pages 6–9
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us