fulltext.study @t Gmail

Catalytic resolution of dl-tryptophan amides using the resting cells of Flavobacterium aquatile ZJB-09211 in a two-phase system

Paper ID Volume ID Publish Year Pages File Format Full-Text
50501 46797 2013 4 PDF Available
Title
Catalytic resolution of dl-tryptophan amides using the resting cells of Flavobacterium aquatile ZJB-09211 in a two-phase system
Abstract

•Ethyl acetate enhanced the activity of amidase and facilitated product separation.•Space-time yield of the bioprocess was improved in this two-phase reaction system.•This methodology may play a pivot role in large scale production of L-tryptophan.

The catalytic activity of Flavobacterium aquatile ZJB-09211 towards the kinetic resolution of DL-tryptophan amides was significantly enhanced by ethyl acetate. A maximum enzyme activity of 5118.62 U/g was obtained under the optimized conditions consisting of a mixture of ethyl acetate and Tris–HCl buffer (30:70). In a scale-up reaction, the tryptophan amide concentration was improved to 200 mM, with 49.85% (e.e. > 99.95%) of the substrate being converted to l-tryptophan. The addition of an organic solvent to the process therefore provided an effective approach for improving the activity of the amidase that could be applied to other amidase-catalyzed bioprocesses.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Amidase; Kinetic resolution; l-Tryptophan; Two-phase system; Ethyl acetate
First Page Preview
Catalytic resolution of dl-tryptophan amides using the resting cells of Flavobacterium aquatile ZJB-09211 in a two-phase system
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Catalysis Communications - Volume 38, 5 August 2013, Pages 31–34
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us