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Microbial synthesis of cis,cis-muconic acid from benzoate by Sphingobacterium sp. mutants

Paper ID Volume ID Publish Year Pages File Format Full-Text
5055 279 2006 6 PDF Available
Title
Microbial synthesis of cis,cis-muconic acid from benzoate by Sphingobacterium sp. mutants
Abstract

The mutants used in this study, Sphingobacterium sp. M4113/M4115, were originated from a wild-type strain GCG, which was isolated from the sewage of a styrene monomer-manufacturing factory. The culturing medium contains appropriate amounts of K2HPO4, (NH4)2SO4, MgSO4·7H2O, Na-succinate, yeast extract and EDTA–FeCl3 complex. The highest conversion yield was obtained with mutant strain M4115, in which more than 0.560 g of cis,cis-muconic acid was accumulated in 28 h from a Na-benzoate solution of 2.0 g/l (∼28% yield). Growth inhibition appeared as the concentration of Na-benzoate exceeded 3.0 g/l. In addition, an increase of 17% and 6% of cis,cis-muconic acid in yield were observed for M4115 and M4113, respectively, as EDTA–FeCl3 complex was employed. It's believed that this complex can enhance the yield of catechol 1,2-dioxygenase, and therefore, the yield of cis,cis-muconic acid according to β-ketoadipate biosynthesis pathway.

Keywords
Aerobic process; Biosynthesis; Bioprocess design; Global environment; cis,cis-Muconic acid; Sphingobacterium sp
First Page Preview
Microbial synthesis of cis,cis-muconic acid from benzoate by Sphingobacterium sp. mutants
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 29, Issues 1–2, 1 April 2006, Pages 35–40
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering